Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
| Type | Pre-packed Column |
|---|---|
| Content And Storage | 4°C to 30°C |
| Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 2 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva Ni Sepharose™ 6 Fast Flow
Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.
| Particle Size | 90μm |
|---|---|
| Buffer | 0.1M HCl, 0.1M NaOH, 8M Urea |
| Flow Rate | 600 cm/hr. |
| Height (Metric) | 250mm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 12 |
| Product Type | Ni Sepharose 6 FF chromatography media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Cross-linked Agarose |
| Max. Pressure | 1 bar |
| Height (English) | 9.84 in. |
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
| Particle Size | 90μm |
|---|---|
| Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
| Flow Rate | 150 to 600cm/hr. |
| Content And Storage | 4° to 30°C |
| pH Range | 2 to 12 |
| Product Type | Ni Sepharose excel chromato graphy media |
| dimMatrix | Ni-Sepharose |
| Support Type | 6% Highly Cross-linked Agarose |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
| Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C |
| Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Material | Polypropylene |
| Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Lauryldimethylamine-N-oxide (LDAO), LC/MS grade
Lauryldimethylamine-N-oxide (LDAO) is a zwitterionic surfactant that possesses unique properties for solubilization and stabilization of biomolecules, particularly membrane proteins.
| CAS | 1643-20-5 |
|---|---|
| Form | Powder |
| Product Type | Lauryldimethylamine-N-oxide |
| Molecular Weight (g/mol) | 229.4 |
| Color | White |
| Purity | ≥99% |
| For Use With (Application) | LC-MS |
| Molecular Formula | C14H31NO |
Thermo Scientific™ Pierce™ ChIP-Grade Protein A/G Plus Agarose
Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.
| Stationary Phase | Protein A/G |
|---|---|
| Type | Agarose |
| Target | IgG Antibody |
| Content And Storage | Upon receipt store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | Pierce |
Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
| Stationary Phase | Metal Chelate, Divalent Cobalt |
|---|---|
| Type | Agarose |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | HisPur |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
| Flow Rate | <20 mL/min. |
|---|---|
| Type | Pre-packed Column |
| Content And Storage | 4°C to 30°C, 20% Ethanol |
| Diameter (English) Inner | 0.6 in. |
| Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
| Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
| pH Range | 3 to 12 |
| Diameter (Metric) Inner | 16 mm |
| Material | Polypropylene |
| Support Type | 6% Cross-linked Agarose |
Thermo Scientific™ SMALP 300
Thermo Scientific SMALP 200 and SMALP 300 are styrene maleic acid (SMA) copolymers designed for the solubilization and stabilization of membrane proteins. These innovative products offer a streamlined approach to membrane protein isolation by combining solubilization and stabilization into a single step.
| Content And Storage | Store at 4°C |
|---|---|
| Sensitivity | Sensitive to light |
| Form | Liquid |
| Product Type | Polymer |
| Reagent Type | Extraction and stabilization |
| pH | 8 |
| Purification Target | Membrane proteins |
| For Use With (Application) | Structure/Function |
| Formula Weight | 10,000 Da |
| Product Line | Thermo Scientific |
Thermo Scientific™ SMALP 200
Thermo Scientific SMALP 200 and SMALP 300 are styrene maleic acid (SMA) copolymers designed for the solubilization and stabilization of membrane proteins. These innovative products offer a streamlined approach to membrane protein isolation by combining solubilization and stabilization into a single step.
| Content And Storage | Store at 4°C |
|---|---|
| Sensitivity | Sensitive to light |
| Form | Liquid |
| Product Type | Polymer |
| Reagent Type | Extraction and stabilization |
| pH | 7.5 |
| Purification Target | Membrane proteins |
| For Use With (Application) | Structure/Function |
| Formula Weight | 6,500 Da |
| Product Line | Thermo Scientific |
Cytiva LabMate™ PD-10 Buffer Reservoirs
Use for high-recovery desalting, buffer exchange and removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium. Cytiva Life Sciences™ LabMate™ PD-10 Buffer Reservoirs provide fast, affordable performance.
Novex™ Protein A
Protein A
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Novex™ Rec Protein A
Rec Protein A
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Intact Virus Precipitation Reagent
Intact Virus Precipitation Reagent wnables fast and efficient enrichment of intact, live SARS-CoV-2 coronavirus from dilute cell culture and virus transport media.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Cytiva StrepTrap XT Chromatography Column
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.
| Height (Metric) | 25 mm |
|---|---|
| Column Type | Affinity Column |
| For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |